Product Description
General MEK (Methionine-Enkephalin) ELISA Kit | ELK9576 | ELK Biotechnology
Alternative Names: Met-enkephalin, Opioid growth factor, OGF
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 75.00 pg/mL
Standard: 8000 pg/mL
Detection Range: 125.00-8000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Signal Transduction
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Methionine-Enkephalin (MEK) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Methionine-Enkephalin (MEK) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methionine-Enkephalin (MEK) in the samples is then determined by comparing the OD of the samples to the standard curve.