Product Description
General NTM (Natamycin) ELISA Kit | ELK8241 | ELK Biotechnology
Alternative Names: Pimaricin; Natacyn
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 1.45 ng/mL
Standard: 300 ng/mL
Detection Range: 4.69-300 ng/mL
Sample Type: biological agents
Assay Lenght: 2h
Research Area: Infectious Diseases
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Natamycin (NTM) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Natamycin (NTM) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Natamycin (NTM) in the samples is then determined by comparing the OD of the samples to the standard curve.