Product Description
General PS (Phosphatidylserine) ELISA Kit | ELK8149 | ELK Biotechnology
Alternative Names: Ptd-L-Ser; PtdSer
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 5.27 ng/mL
Standard: 1200 ng/mL
Detection Range: 18.75-1200 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Neuro science;Developmental science;Nutrition metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Phosphatidylserine (PS) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phosphatidylserine (PS) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphatidylserine (PS) in the samples is then determined by comparing the OD of the samples to the standard curve.