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Product Description
General TCA (Taurocholic Acid) ELISA Kit | ELK8173 | ELK Biotechnology
Alternative Names: Cholaic Acid; Cholyltaurine; Acidum Cholatauricum
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 1.41 ng/mL
Standard: 300 ng/mL
Detection Range: 4.69-300 ng/mL
Sample Type: Biological agents
Assay Lenght: 2h
Research Area: Signal transduction
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Taurocholic Acid (TCA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Taurocholic Acid (TCA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Taurocholic Acid (TCA) in the samples is then determined by comparing the OD of the samples to the standard curve.
