Product Description
General VB1 (Vitamin B1) ELISA Kit | ELK7916 | ELK Biotechnology
Alternative Names: Thiamine; Thiamin; Aneurine
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 1.63 ng/mL
Standard: 300 ng/mL
Detection Range: 4.69-300 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Nutrition metabolism;Hormone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Vitamin B1 (VB1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vitamin B1 (VB1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin B1 (VB1) in the samples is then determined by comparing the OD of the samples to the standard curve.