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Product Description
Dog AMH (Anti-Mullerian Hormone) ELISA Kit | ELK9220 | ELK Biotechnology
Alternative Names: MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance
Species: Dog
Assay Type: Competitive Inhibition
Sensitivity: 25.4 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Endocrinology;Reproductive science;Hormone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Anti-Mullerian Hormone (AMH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anti-Mullerian Hormone (AMH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Mullerian Hormone (AMH) in the samples is then determined by comparing the OD of the samples to the standard curve.
