Product Description
Human LTE4 (Cysteinyl Leukotrienes E4) ELISA Kit | ELK9554 | ELK Biotechnology
Alternative Names: LTE4 ELISA Kit; Cysteinyl Leukotrienes E4 ELISA Kit
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 5.25 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Immunology; Biochemicals
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cysteinyl Leukotrienes E4 (LTE4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cysteinyl Leukotrienes E4 (LTE4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteinyl Leukotrienes E4 (LTE4) in the samples is then determined by comparing the OD of the samples to the standard curve.