Euro
USD
British Pound
NULL
Product Description
Human NEFL (Neurofilament, Light Polypeptide) ELISA Kit | ELK5535 | ELK Biotechnology
Alternative Names: CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 46.4 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Neuro science
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Neurofilament, Light Polypeptide (NEFL) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neurofilament, Light Polypeptide (NEFL) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurofilament, Light Polypeptide (NEFL) in the samples is then determined by comparing the OD of the samples to the standard curve.
