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Product Description
Human PI (Proinsulin) ELISA Kit | ELK5406 | ELK Biotechnology
Alternative Names: INS; Pro-Insulin; Preproinsulin
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 5.13 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Endocrinology;Hormone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Proinsulin (PI) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Proinsulin (PI) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proinsulin (PI) in the samples is then determined by comparing the OD of the samples to the standard curve.
