Product Description
Human Tb4Y (Thymosin Beta 4, Y-Chromosomal) ELISA Kit | ELK4395 | ELK Biotechnology
Alternative Names: TMSB4Y; Thymosin, Beta 4, Y-Linked
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 42.2 ng/mL
Standard: 10000 ng/mL
Detection Range: 156.25-10000 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 2h
Research Area: Endocrinology
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thymosin Beta 4, Y-Chromosomal (Tb4Y) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thymosin Beta 4, Y-Chromosomal (Tb4Y) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thymosin Beta 4, Y-Chromosomal (Tb4Y) in the samples is then determined by comparing the OD of the samples to the standard curve.