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Product Description
Human Total cholesterol (TC) ELISA Kit | ELK8420 | ELK Biotechnology
Alternative Names: TC
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 2.63 μg/mL
Standard: 500 μg/mL
Detection Range: 7.82-500 μg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Metabolic pathway;Hepatology;Gastroenterology;Nutrition metabolism;Hormone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with TC (Total cholesterol ) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TC (Total cholesterol ) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TC (Total cholesterol ) in the samples is then determined by comparing the OD of the samples to the standard curve.
