Product Description
Human TSHRAb (Thyroid Stimulating Hormone Receptor Antiboby) ELISA Kit | ELK9540 | ELK Biotechnology
Alternative Names: TSHRAb
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 0.85 mIU/mL
Standard: 100mIU/mL
Detection Range: 1.57-100mIU/mL
Sample Type: Serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Signal Transduction; Neuroscience
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyroid Stimulating Hormone Receptor Antiboby (TSHRAb) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyroid Stimulating Hormone Receptor Antiboby (TSHRAb) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroid Stimulating Hormone Receptor Antiboby (TSHRAb) in the samples is then determined by comparing the OD of the samples to the standard curve.