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Product Description
Human ASMA (Anti-Smooth Muscle Antibody) ELISA Kit | ELK9447 | ELK Biotechnology
Alternative Names: ASM Ab; Anti Smooth Muscle Antibody
Species: Human
Assay Type: Sandwich
Sensitivity: 3.2 ng/mL
Standard: 500 ng/mL
Detection Range: 7.82-500 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anti-Smooth Muscle Antibody (ASMA) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anti-Smooth Muscle Antibody (ASMA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anti-Smooth Muscle Antibody (ASMA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Smooth Muscle Antibody (ASMA) in the samples is then determined by comparing the OD of the samples to the standard curve.
