Product Description
Human aZGP1 (Alpha-2-Glycoprotein 1, Zinc Binding) ELISA Kit | ELK6240 | ELK Biotechnology
Alternative Names: ZA2G; ZAG
Species: Human
Assay Type: Sandwich
Sensitivity: 1.8 ng/mL
Standard: 300 ng/mL
Detection Range: 4.69-300 ng/mL
Sample Type: serum, plasma, urine, saliva, seminal plasma, sweat and other biological fluids
Assay Lenght: 3.5h
Research Area: Metabolic pathway
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) in the samples is then determined by comparing the OD of the samples to the standard curve.