Product Description
Human BMP5 (Bone Morphogenetic Protein 5) ELISA Kit | ELK5119 | ELK Biotechnology
Alternative Names: BMP 5; BMP-5; Bmp5; BMP5
Species: Human
Assay Type: Sandwich
Sensitivity: 5.3 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction;Tumor immunity;Developmental science;Bone metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 5 (BMP5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 5 (BMP5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 5 (BMP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone Morphogenetic Protein 5 (BMP5) in the samples is then determined by comparing the OD of the samples to the standard curve.