Product Description
Human C3d (ComplementFragment 3d) ELISA Kit | ELK9598 | ELK Biotechnology
Alternative Names: C3d
Species: Human
Assay Type: Sandwich
Sensitivity: 4.69 ng/mL
Standard: 500 ng/mL
Detection Range: 7.81-500 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Immunology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ComplementFragment 3d (C3d) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ComplementFragment 3d (C3d) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ComplementFragment 3d (C3d), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ComplementFragment 3d (C3d) in the samples is then determined by comparing the OD of the samples to the standard curve.