Product Description
Human CHST9 (Carbohydrate Sulfotransferase 9) ELISA Kit | ELK3946 | ELK Biotechnology
Alternative Names: GALNAC4ST-2; GALNAC-4-ST2; N-acetylgalactosamine-4-O-sulfotransferase 2; GalNAc-4-O-sulfotransferase 2
Species: Human
Assay Type: Sandwich
Sensitivity: 0.25 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbohydrate Sulfotransferase 9 (CHST9) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Carbohydrate Sulfotransferase 9 (CHST9) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbohydrate Sulfotransferase 9 (CHST9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbohydrate Sulfotransferase 9 (CHST9) in the samples is then determined by comparing the OD of the samples to the standard curve.