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Product Description
Human COL1A1 (Pro-Collagen I alpha 1) ELISA Kit | ELK9188 | ELK Biotechnology
Alternative Names: Alpha 1 type I collagen; Alpha 2 type I collagen; alpha 2 type I procollagen; alpha 2 (I) procollagen; alpha 2 (I) -collagen; Alpha-1 type I collagen; alpha1 (I) procollagen
Species: Human
Assay Type: Sandwich
Sensitivity: 15 pg/mL
Standard: 2500 pg/mL
Detection Range: 39.07-2500 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal Transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pro-Collagen I alpha 1 (COL1A1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pro-Collagen I alpha 1 (COL1A1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pro-Collagen I alpha 1 (COL1A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pro-Collagen I alpha 1 (COL1A1) in the samples is then determined by comparing the OD of the samples to the standard curve.
