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Product Description
Human COL5a2 (Collagen Type V Alpha 2) ELISA Kit | ELK3965 | ELK Biotechnology
Alternative Names: COL5-A2; Collagen Alpha-2 (V) chain; AB Collagen
Species: Human
Assay Type: Sandwich
Sensitivity: 0.125 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type V Alpha 2 (COL5a2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type V Alpha 2 (COL5a2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type V Alpha 2 (COL5a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type V Alpha 2 (COL5a2) in the samples is then determined by comparing the OD of the samples to the standard curve.
