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Product Description
Human CRHR1 (Corticotropin Releasing Hormone Receptor 1) ELISA Kit | ELK4803 | ELK Biotechnology
Alternative Names: CRF-R; CRF1; CRFR1; CRH-R1h; CRHR; CRHR1f; Corticotropin-releasing factor receptor 1
Species: Human
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Endocrinology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Corticotropin Releasing Hormone Receptor 1 (CRHR1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Corticotropin Releasing Hormone Receptor 1 (CRHR1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Corticotropin Releasing Hormone Receptor 1 (CRHR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Corticotropin Releasing Hormone Receptor 1 (CRHR1) in the samples is then determined by comparing the OD of the samples to the standard curve.
