Product Description
Human EGFL6 (EGF Like Domain Protein, Multiple 6) ELISA Kit | ELK6160 | ELK Biotechnology
Alternative Names: MAEG; MAM And EGF Domain Containing; Epidermal growth factor-like protein 6
Species: Human
Assay Type: Sandwich
Sensitivity: 35.95 pg/mL
Standard: 6000 pg/mL
Detection Range: 93.75-6000 pg/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF Like Domain Protein, Multiple 6 (EGFL6) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to EGF Like Domain Protein, Multiple 6 (EGFL6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGF Like Domain Protein, Multiple 6 (EGFL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EGF Like Domain Protein, Multiple 6 (EGFL6) in the samples is then determined by comparing the OD of the samples to the standard curve.