Product Description
Human FAM19A5 (Protein FAM19A5) ELISA Kit | ELK8907 | ELK Biotechnology
Alternative Names: Chemokine like protein TAFA 5; FAM19A5; Protein FAM19A5; QLLK5208; TAFA 5; TAFA5; UNQ5208
Species: Human
Assay Type: Sandwich
Sensitivity: 26.5 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 3.5h
Research Area: Autistic Disorder
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein FAM19A5 (FAM19A5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein FAM19A5 (FAM19A5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein FAM19A5 (FAM19A5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein FAM19A5 (FAM19A5) in the samples is then determined by comparing the OD of the samples to the standard curve.