Product Description
Human FLOT1 (Flotillin 1) ELISA Kit | ELK5386 | ELK Biotechnology
Alternative Names: FLOT 1; FLOT1; Flotillin-1; Flotillin1
Species: Human
Assay Type: Sandwich
Sensitivity: 1.18 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Metabolic pathway
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Flotillin 1 (FLOT1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Flotillin 1 (FLOT1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Flotillin 1 (FLOT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Flotillin 1 (FLOT1) in the samples is then determined by comparing the OD of the samples to the standard curve.