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Product Description
Human GZMH (Granzyme H) ELISA Kit | ELK6670 | ELK Biotechnology
Alternative Names: CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2; Cathepsin G-Like 2, Protein h-CCPX; Cytotoxic T-lymphocyte proteinase; Cytotoxic serine protease C
Species: Human
Assay Type: Sandwich
Sensitivity: 0.61 pg/mL
Standard: 100 pg/mL
Detection Range: 1.57-100 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granzyme H (GZMH) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Granzyme H (GZMH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Granzyme H (GZMH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Granzyme H (GZMH) in the samples is then determined by comparing the OD of the samples to the standard curve.
