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Product Description
Human IFA (Intrinsic Factor Antibody) ELISA Kit | ELK9327 | ELK Biotechnology
Alternative Names: Gastric Intrinsic Factor; Gastric intrinsic factor (vitamin B synthesis) ; GIF; TCN3; TCM3
Species: Human
Assay Type: Sandwich
Sensitivity: 29 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction; Metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Intrinsic Factor Antibody (IFA) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Intrinsic Factor Antibody (IFA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Intrinsic Factor Antibody (IFA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Intrinsic Factor Antibody (IFA) in the samples is then determined by comparing the OD of the samples to the standard curve.
