Product Description
Human KPNa2 (Karyopherin Alpha 2) ELISA Kit | ELK4114 | ELK Biotechnology
Alternative Names: IPOA1; QIP2; RCH1; SRP1alpha; RAG Cohort 1; Importin Alpha 1; RAG cohort protein 1; SRP1-alpha
Species: Human
Assay Type: Sandwich
Sensitivity: 52 pg/mL
Standard: 8000 pg/mL
Detection Range: 125-8000 pg/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Immune molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Karyopherin Alpha 2 (KPNa2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Karyopherin Alpha 2 (KPNa2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Karyopherin Alpha 2 (KPNa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Karyopherin Alpha 2 (KPNa2) in the samples is then determined by comparing the OD of the samples to the standard curve.