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Product Description
Human LPHN3 (Latrophilin 3) ELISA Kit | ELK4018 | ELK Biotechnology
Alternative Names: CIRL3; LEC3; Calcium-independent alpha-latrotoxin receptor 3; Lectomedin-3
Species: Human
Assay Type: Sandwich
Sensitivity: 0.114 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Latrophilin 3 (LPHN3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Latrophilin 3 (LPHN3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Latrophilin 3 (LPHN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Latrophilin 3 (LPHN3) in the samples is then determined by comparing the OD of the samples to the standard curve.
