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Product Description
Human MCPIP (Monocyte Chemoattractant Protein-induced Protein 1) ELISA Kit | ELK8584 | ELK Biotechnology
Alternative Names: Ribonuclease ZC3H12A; MCP-induced protein 1; Zinc finger CCCH domain-containing protein 12A; MCPIP1
Species: Human
Assay Type: Sandwich
Sensitivity: 1.5 pg/mL
Standard: 250 pg/mL
Detection Range: 3.91-250 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monocyte Chemoattractant Protein-induced Protein 1 (MCPIP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Monocyte Chemoattractant Protein-induced Protein 1 (MCPIP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Monocyte Chemoattractant Protein-induced Protein 1 (MCPIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Monocyte Chemoattractant Protein-induced Protein 1 (MCPIP) in the samples is then determined by comparing the OD of the samples to the standard curve.
