Euro
USD
British Pound
NULL
Product Description
Human PLD2 (Phospholipase D2) ELISA Kit | ELK4093 | ELK Biotechnology
Alternative Names: PLD1C; Choline Phosphatase 2; Phosphatidylcholine-hydrolyzing phospholipase D2
Species: Human
Assay Type: Sandwich
Sensitivity: 0.55 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Metabolic pathway
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase D2 (PLD2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phospholipase D2 (PLD2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase D2 (PLD2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase D2 (PLD2) in the samples is then determined by comparing the OD of the samples to the standard curve.
