Product Description
Human PNX-20 (Phoenixin-20) ELISA Kit | ELK8899 | ELK Biotechnology
Alternative Names: Small integral membrane protein; Mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein of 7 kDa; MITRAC7; Phoenixin-14; PNX-14; Phoenixin-20; PNX-20; SMIM20; C4orf52
Species: Human
Assay Type: Sandwich
Sensitivity: 7.4 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phoenixin-20 (PNX-20) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phoenixin-20 (PNX-20) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phoenixin-20 (PNX-20), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phoenixin-20 (PNX-20) in the samples is then determined by comparing the OD of the samples to the standard curve.