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Product Description
Human PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent 1A) ELISA Kit | ELK4276 | ELK Biotechnology
Alternative Names: PP2C-ALPHA; PP2CA; Phosphatase 2C Alpha; Protein Phosphatase 1A (Formerly 2C) Magnesium-Dependent, Alpha
Species: Human
Assay Type: Sandwich
Sensitivity: 0.123 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) in the samples is then determined by comparing the OD of the samples to the standard curve.
