Product Description
Human RAD51B (DNA repair protein RAD51 homolog 2) ELISA Kit | ELK9348 | ELK Biotechnology
Alternative Names: hREC2; MGC34245; R51H2; RAD51 homolog B; RAD51 like protein 1
Species: Human
Assay Type: Sandwich
Sensitivity: 0.115 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Epigenetics and Nuclear Signaling
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA repair protein RAD51 homolog 2 (RAD51B) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DNA repair protein RAD51 homolog 2 (RAD51B) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNA repair protein RAD51 homolog 2 (RAD51B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DNA repair protein RAD51 homolog 2 (RAD51B) in the samples is then determined by comparing the OD of the samples to the standard curve.