Product Description
Human SCARB2 (Scavenger Receptor Class B Member 2) ELISA Kit | ELK8459 | ELK Biotechnology
Alternative Names: SR BII; SRB2; Scavenger receptor class B member 2; Lysosome membrane protein 2; Lysosome membrane protein II; LIMP 2
Species: Human
Assay Type: Sandwich
Sensitivity: 0.229 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: CD & Adhesion molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Scavenger Receptor Class B Member 2 (SCARB2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Scavenger Receptor Class B Member 2 (SCARB2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Scavenger Receptor Class B Member 2 (SCARB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Scavenger Receptor Class B Member 2 (SCARB2) in the samples is then determined by comparing the OD of the samples to the standard curve.