Product Description
Human TIGIT (T-Cell Immunoreceptor With Ig And ITIM Domains Protein) ELISA Kit | ELK7357 | ELK Biotechnology
Alternative Names: VSIG9; VSTM3; V-Set And Immunoglobulin Domain Containing 9; V-Set And Transmembrane Domain Containing 3
Species: Human
Assay Type: Sandwich
Sensitivity: 0.133 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) in the samples is then determined by comparing the OD of the samples to the standard curve.