Product Description
Human TRIC (Tricellulin) ELISA Kit | ELK7567 | ELK Biotechnology
Alternative Names: MARVELD2; DFNB49; MARVD2; MRVLDC2; MARVEL Domain Containing Protein 2; Deafness, Autosomal Recessive 49
Species: Human
Assay Type: Sandwich
Sensitivity: 0.125 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tricellulin (TRIC) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tricellulin (TRIC) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tricellulin (TRIC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tricellulin (TRIC) in the samples is then determined by comparing the OD of the samples to the standard curve.