Euro
USD
British Pound
NULL
Product Description
Human AAP (Alanine Aminopeptidase) ELISA Kit | ELK2996 | ELK Biotechnology
Alternative Names: CD13; ALAP; P150; ANPEP; APN; LAP1; PEPN; Gp150; Aminopeptidase M; Microsomal Aminopeptidase; Alanyl Membrane Aminopeptidase; Aminopeptidase N
Species: Human
Assay Type: Sandwich
Sensitivity: 0.57 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase;Kidney biomarker
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alanine Aminopeptidase (AAP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alanine Aminopeptidase (AAP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alanine Aminopeptidase (AAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alanine Aminopeptidase (AAP) in the samples is then determined by comparing the OD of the samples to the standard curve.
