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Product Description
Human ASAH1 (N-Acylsphingosine Amidohydrolase 1) ELISA Kit | ELK2966 | ELK Biotechnology
Alternative Names: AC; ASAH; PHP; PHP32; Acid Ceramidase; Acylsphingosine deacylase; Putative 32 kDa heart protein
Species: Human
Assay Type: Sandwich
Sensitivity: 0.63 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Acylsphingosine Amidohydrolase 1 (ASAH1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to N-Acylsphingosine Amidohydrolase 1 (ASAH1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Acylsphingosine Amidohydrolase 1 (ASAH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Acylsphingosine Amidohydrolase 1 (ASAH1) in the samples is then determined by comparing the OD of the samples to the standard curve.
