Product Description
Human C1qC (Complement Component 1, Q Subcomponent C) ELISA Kit | ELK3574 | ELK Biotechnology
Alternative Names: C1QG; Complement Component 1 q subcomponent, Gamma Polypeptide
Species: Human
Assay Type: Sandwich
Sensitivity: 13.7 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Immune molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 1, Q Subcomponent C (C1qC) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Complement Component 1, Q Subcomponent C (C1qC) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 1, Q Subcomponent C (C1qC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 1, Q Subcomponent C (C1qC) in the samples is then determined by comparing the OD of the samples to the standard curve.