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Product Description
Human CAM (Calmodulin) ELISA Kit | ELK2014 | ELK Biotechnology
Alternative Names: CALcium MODULated ProteIN
Species: Human
Assay Type: Sandwich
Sensitivity: 0.072 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction;Metabolic pathway;Apoptosis;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calmodulin (CAM) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Calmodulin (CAM) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calmodulin (CAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calmodulin (CAM) in the samples is then determined by comparing the OD of the samples to the standard curve.
