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Product Description
Human COL1a2 (Collagen Type I Alpha 2) ELISA Kit | ELK1985 | ELK Biotechnology
Alternative Names: COL1-A2; OI4; Collagen Alpha-2 (I) chain; osteogenesis imperfecta type IV; Collagen Of Skin, Tendon And bone, Alpha-2 Chain
Species: Human
Assay Type: Sandwich
Sensitivity: 6.3 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Metabolic pathway;Bone metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type I Alpha 2 (COL1a2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type I Alpha 2 (COL1a2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type I Alpha 2 (COL1a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type I Alpha 2 (COL1a2) in the samples is then determined by comparing the OD of the samples to the standard curve.
