Product Description
Human DPP10 (Dipeptidyl Peptidase 10) ELISA Kit | ELK3067 | ELK Biotechnology
Alternative Names: DPL2; DPPY; DPRP3; Dipeptidylpeptidase 10 (Non-Functional) ; Dipeptidyl peptidase IV-related protein 3; Dipeptidyl peptidase X; Dipeptidyl peptidase-like protein 2
Species: Human
Assay Type: Sandwich
Sensitivity: 0.116 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dipeptidyl Peptidase 10 (DPP10) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dipeptidyl Peptidase 10 (DPP10) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dipeptidyl Peptidase 10 (DPP10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dipeptidyl Peptidase 10 (DPP10) in the samples is then determined by comparing the OD of the samples to the standard curve.