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Product Description
Human FGFR2 (Fibroblast Growth Factor Receptor 2) ELISA Kit | ELK3090 | ELK Biotechnology
Alternative Names: CD332; BEK; BFR1; CEK3; CFD1; ECT1; JWS; K-SAM; KGFR; TK14; Bacteria-Expressed Kinase, Keratinocyte Growth Factor Receptor; Craniofacial Dysostosis 1; Crouzon Syndrome
Species: Human
Assay Type: Sandwich
Sensitivity: 12.2 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor Receptor 2 (FGFR2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fibroblast Growth Factor Receptor 2 (FGFR2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor Receptor 2 (FGFR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor Receptor 2 (FGFR2) in the samples is then determined by comparing the OD of the samples to the standard curve.
