Product Description
Human GC (Glucagon) ELISA Kit | ELK2159 | ELK Biotechnology
Alternative Names: Carbohydrate-Rich Portion of Platelet Membrane Glycoprotein Ib Alpha Polypeptide
Species: Human
Assay Type: Sandwich
Sensitivity: 7.3 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Plasma
Assay Lenght: 3.5h
Research Area: Signal transduction;Hematology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucagon (GC) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glucagon (GC) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucagon (GC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucagon (GC) in the samples is then determined by comparing the OD of the samples to the standard curve.