Product Description
Human GKN2 (Gastrokine 2) ELISA Kit | ELK3610 | ELK Biotechnology
Alternative Names: TFIZ1; BLOT; GDDR; VLTI465; Blottin; Down Regulated In Gastric Cancer; Trefoil factor interactions (z) 1
Species: Human
Assay Type: Sandwich
Sensitivity: 6.1 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Cytokine
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gastrokine 2 (GKN2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gastrokine 2 (GKN2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gastrokine 2 (GKN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gastrokine 2 (GKN2) in the samples is then determined by comparing the OD of the samples to the standard curve.