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Product Description
Human GP39 (Glycoprotein 39, Cartilage) ELISA Kit | ELK1991 | ELK Biotechnology
Alternative Names: CHI3L1; CHI3-L1; YKL40; Chondrex; Chitinase 3-Like 1; Chondrocyte Protein YKL40; 39 kDa synovial protein
Species: Human
Assay Type: Sandwich
Sensitivity: 13.5 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Metabolic pathway;Infection immunity;Bone metabolism;Rheumatology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycoprotein 39, Cartilage (GP39) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glycoprotein 39, Cartilage (GP39) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycoprotein 39, Cartilage (GP39), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycoprotein 39, Cartilage (GP39) in the samples is then determined by comparing the OD of the samples to the standard curve.
