Product Description
Human IRF9 (Interferon Regulatory Factor 9) ELISA Kit | ELK3224 | ELK Biotechnology
Alternative Names: ISGF3G; ISGF3; p48; Interferon-Stimulated Transcription Factor 3, Gamma 48kDa; IFN-alpha-responsive transcription factor subunit; Interferon-stimulated gene factor 3 gamma
Species: Human
Assay Type: Sandwich
Sensitivity: 0.048 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Immune molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Regulatory Factor 9 (IRF9) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interferon Regulatory Factor 9 (IRF9) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Regulatory Factor 9 (IRF9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Regulatory Factor 9 (IRF9) in the samples is then determined by comparing the OD of the samples to the standard curve.