Product Description
Human ITIH1 (Inter Alpha-Globulin Inhibitor H1) ELISA Kit | ELK3442 | ELK Biotechnology
Alternative Names: H1P; IATIH; ITIH; IGHEP1; Serum-derived hyaluronan-associated protein; Inter-alpha-trypsin inhibitor complex component III
Species: Human
Assay Type: Sandwich
Sensitivity: 0.071 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Metabolic pathway
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inter Alpha-Globulin Inhibitor H1 (ITIH1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Inter Alpha-Globulin Inhibitor H1 (ITIH1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inter Alpha-Globulin Inhibitor H1 (ITIH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inter Alpha-Globulin Inhibitor H1 (ITIH1) in the samples is then determined by comparing the OD of the samples to the standard curve.