Product Description
Human KLK11 (Kallikrein 11) ELISA Kit | ELK2094 | ELK Biotechnology
Alternative Names: TLSP; hK11; Hippostatin; Kallikrein-Related Peptidase 11; Serine protease 20; Trypsin-like protease
Species: Human
Assay Type: Sandwich
Sensitivity: 0.053 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase;Tumor immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kallikrein 11 (KLK11) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kallikrein 11 (KLK11) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kallikrein 11 (KLK11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kallikrein 11 (KLK11) in the samples is then determined by comparing the OD of the samples to the standard curve.