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Product Description
Human MCP (Membrane Cofactor Protein) ELISA Kit | ELK1836 | ELK Biotechnology
Alternative Names: CD46; MIC10; TLX; TRA2; Trophoblast leukocyte common antigen; CD46 Molecule, Complement Regulatory Protein; CD46 Molecule, Complement Regulatory protein
Species: Human
Assay Type: Sandwich
Sensitivity: 4.8 pg/mL
Standard: 800 pg/mL
Detection Range: 12.5-800 pg/mL
Sample Type: Serum, plasma, urine, saliva and other biological fluids
Assay Lenght: 3.5h
Research Area: CD & Adhesion molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Membrane Cofactor Protein (MCP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Membrane Cofactor Protein (MCP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Membrane Cofactor Protein (MCP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Membrane Cofactor Protein (MCP) in the samples is then determined by comparing the OD of the samples to the standard curve.
