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Product Description
Human NCAM (Neural Cell Adhesion Molecule) ELISA Kit | ELK1813 | ELK Biotechnology
Alternative Names: CD56; NCAM1; MSK39; N-CAM-1
Species: Human
Assay Type: Sandwich
Sensitivity: 0.63 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Neuro science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neural Cell Adhesion Molecule (NCAM) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neural Cell Adhesion Molecule (NCAM) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neural Cell Adhesion Molecule (NCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neural Cell Adhesion Molecule (NCAM) in the samples is then determined by comparing the OD of the samples to the standard curve.
